AFC Summer 2012 meeting

AFC Summer 2012

Held at the Brighton and Sussex Medical School, University of Sussex

July 5th-6th 2012

Scientific Programme

Session 1: Brighton and Sussex Medical School and University ofSussex Symposium

Genetic susceptibility to M.tuberculosis
Melanie Newport

Interactions between a novel transcriptional activator and RNA polymerase
Mark Paget

Flow-cytometry in the diagnosis of active TB
Florian Kern

DNA repair in mycobacteria
Aidan Doherty

The effect of growth rate on the efficacy of isoniazid against Mycobacterium tuberculosis
Alice Marriott, Jon Allnutt, Kim Hatch, Catherine Arnold, Tim McHugh, Philip Marsh, Joanna Bacon, HPA Microbiology Services, Porton Down, Salisbury

Session 2: Guest speaker

Host-pathogen interactions in TB
Olivier Neyrolles. CNRS, Toulouse

Session 3: Offered papers

Evaluating BCG and novel TB vaccine candidates with a human BCG challenge model
Joel Meyer, S Harris, I Satti, A Minassian, H Fletcher, H McShane, Jenner Institute, Oxford University

Does biofilm growth of Mycobacterium tuberculosis replicate features of the phenotypes observed in tuberculous sputum?
Abdulwhab Binjomah, Andrew Bell, Su-Min Lee, Galina Mukamolova, Natalie Garton and Mike Barer, Department of Infection Immunity and Inflammation, University of Leicester

The role of peptidoglycan glycolylation in mycobacterial dormancy and resuscitation
Jessica Loraine, Obolbek Turapov, Jacob Biboy, Waldemar VollmerandGalina Mukamolova, Infection, Immunity, Inflammation Department and Chemistry Department, University of Leicester

Transcriptional profiling of disease-induced host responses in bovine tuberculosis
Elihu Aranday-Cortes, Philip J. Hogarth, Daryan A. Kaveh, Adam O. Whelan, Bernardo Villarreal-Ramos, Ajit Lalvani, H. Martin Vordermeier, Animal Heath & Veterinary Laboratories Agency, Surrey

Measuring phosphorylation of the metabolic regulator GarA in living mycobacteria
Barbara Rieck, A. R. Bottrill, H M O’Hare, Department of Infection, Immunity, and Inflammation, University of Leicester

Session 3: Offered papers

Genome-wide mapping of the GlnR regulon in Mycobacterium smegmatis
Victoria Jenkins, Brian Robertson and Kerstin Williams, MRC CMBI, Flowers Building, Imperial College London, South Kensington, London

Identification of novel Mt-GuaB2 inhibitor series active against M. tuberculosis
Veeraraghavan Usha, Judith V. Hobrath, Sudagar S. Gurcha, Robert C. Reynoldsand Gurdyal S. Besra, School of Biosciences, University of Birmingham, Edgbaston, Birmingham

ATP-dependent Mur ligases in the biogenesis of cell wall peptidoglycan in Mycobacterium tuberculosis: novel targets for anti-TB drug discovery
Tulika Munshi, Antima Gupta, Dimitrios Evangelopolous, Juan David Guzman, Nicholas H. Keep, Simon Gibbons and Sanjib Bhakta, Mycobacteria Research Laboratory, Department of Biological Sciences, Institute of Structural & Molecular Biology, Birkbeck, University of London

Impaired host immune system favours tuberculosis induced by Mycobacterium africanum but not Mycobacterium tuberculosis
Leopold. D. Tientcheu, J. Sutherland, B. de Jong, S. Smith, H. M. Dockrell, M. O. Ota, Vaccinology Theme, Medical Research Council Unit The Gambia, and Department of Immunology and Infection, Faculty of Infectious and Tropical Disease, London School of Hygiene & Tropical Medicine

AFC Winter 2012 meeting

AFC Winter 2012

Held at Imperial College, London

January 6th 2012

Scientific Programme

Session 1

Independent impact of tuberculosis disease stage and HIV co-infection on Mycobacterium tuberculosis (MTB)-specific cellular immunity
Katrina M Pollock, Montamat-Sicotte D, Cooke G, Kapembwa M, Kon O M, Taylor G P and Lalvani A. Tuberculosis Research Unit, Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London

Mycobacterial Growth Indicator Tube (MGIT) assay as a correlate of protection against TB
Leanne Marsay, R. Tanner, H. Poyntz, K. Griffiths, E. Stylianou, M. Kostov, H. Fletcher, S. Sharpe, H. McShane. The Jenner Institute, University of Oxford, Oxford, UK

Increased diagnostic yield of sputum samples for Mycobacterium tuberculosis by loop mediated amplification (LAMP) compared to sputum microscopy in Kerbala, Iraq
Mohanad Ahmed, Hassan Abood, Natalie Garton and Mike Barer.West Euphrates Research Unit, Kerbala University, Kerbala, Iraq

Evaluation of the influence of the route of administration in the immunogenicity of Ad5-Ag85A as booster in BCG vaccinated cattle
Adam Whelan, Pinar Court, Glyn Hewinson, Xhou Zing, Derek Clifford, Martin Vordermeier, Bernardo Villarreal-Ramos. TB Research Group, Animal Health and Veterinary Laboratories Agency, Surrey

A TetR-type regulator represses the production of oleic acid in mycobacteria
Mahmudul Hasan Sikder, Mike Withers, Ricardo Balhana, Neil Stoker and Sharon Kendall. Department of Pathology and Infectious Diseases, The Royal Veterinary College, London

Session 2

Vitamin D accelerates resolution of inflammatory responses during tuberculosis treatment
Anna KCoussens, Hanifa Y, Nikolayevskyy V, Elkington PT, Islam K, Timms PM, Venton TR, Bothamley GH, Packe GE, Darmalingam M, Davidson RN, Milburn HJ, Baker LV, Barker RD, Mein CA, Bhaw-Rosun L, Nuamah R, Young DB, Drobniewski FA, Griffiths CJ, Wilkinson RJ, Martineau AR. Division of Mycobacterial Research, MRC National Institute of Medical Research, London

Expression of the two ClpP protease subunits in Mycobacterium tuberculosis
Yoann Personne, Amanda C. Brown, Tanya Parish. Barts and London School of Medicine and Dentistry, Queen Mary University of London

Phthiocerol dimycocerosate waxes are potential biomarkers for the rapid diagnosis of tuberculosis in sputum
David E. Minnikin, Ruth McNerney,Denise M. O’ Sullivan, Simona C. Nicoara, Elizabeth L. Corbett, Reggie Mutetwa, Stanley Mungofa, Oona Y-C. Lee, Max W. Bardwell, Geraint H. Morgan. School of Biosciences, University of Birmingham

Systems pharmacology approach to antimycobacterial drug discovery. Luiz Pedro S. de Carvalho and Douglas Young. Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London

Serodiagnosis of tuberculosis: due to change track
Juraj Ivanyi. Guy’s Campus of Kings College London

AFC Summer 2011 meeting

AFC Summer 2011

Held at the University of Surrey

July 8th 2011

Scientific Programme

Session 1: Surrey University

Interrogating transcriptomic data with a genome-scale model of metabolism: what does M. tuberculosis eat in vivo?
Bhushan Bonde, Dany Beste, Emma Laing, Andrzej M. Kierzek, Johnjoe McFadden

Cell death fates in Mycobacteruim tuberculosis infected             macrophages are influenced by multiplicity of infection, virulence, and strain characteristics
Rachel E. Butler, Priscille Brodin, Jichan Jang, Mi-Seon Jang, Hilde Smith, Jelle Thole, Brigitte Gicquel, Olivier Neyrolles and Graham R Stewart

Session 2

Exploring the therapeutic potential of a novel human IgA antibody against tuberculosis
Rajko Reljic, Sucharitha Balu, Jenny Woof and Juraj Ivanyi. St George’s, University of London

Genome Scale Modelling of Mycobacterium bovis and Mycobacterium bovis Bacille Calmette-Guérin
Emma Lofthouse, Paul Wheeler, Andrzej Kierzek, Dany Beste, Tom Mendum and Johnjoe McFadden. Tuberculosis Research Group, Animal Health and Veterinary Laboratories Agency,Weybridge

An evaluation of the safety and immunogenicity of MVA85A delivered by aerosol to the lungs of macaques
A.D. White, K. Gooch, L. Sibley, M.J. Dennis, G. Betts, A. Reyes-Sandoval, A. Williams, P.D. Marsh, H. McShane, S.A. Sharpe. Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury

Broad cytokine/chemokine response in peripheral blood stimulated with heparin-binding haemagglutinin following BCG vaccination of UK infants
Steven G. Smith, Sophie Lecher, Rose Blitz, Camille Locht, Hazel M. Dockrell. Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London

The use of low temperature incubation to screen for whole cell activity of drugs against persisters
D.Coleman, D.A.Mitchison, S Waddell, St Georges, University of London

Session 3

Mycobacterium leprae upregulates human beta defensins in cutaneous leprosy Type 1 Reactions
Anna L. Cogen, Steven L. Walker, Chrissy Roberts, Sushmita Dhakal, Rachel A. Hawksworth, Kishori Mahat , Diana N.J. Lockwood. Faculty of Infectious Tropical Diseases, Department of Clinical Research, London School of Hygiene and Tropical Medicine, London

Mycobacterium tuberculosis lineage influences innate immune response and is associated with distinct cell envelope lipids
Nitya Krishnan, Wladimir Malaga, Maxine Caws, Douglas.B.Young, Brian D. Robertson, Christophe Guilhot, Guy.E.Thwaites. Centre for Molecular Microbiology and Infection, Imperial College, London

The C-Terminal Domain of the Ethambutol target Mt-EmbC is a Lectin-like Carbohydrate Binding Module
Luke J. Alderwick, Georgina S. Lloyd, Hemza Ghadbane, John W. May, Apoorva Bhatt, Lothar Eggeling, Klaus Fütterer, Gurdyal S. Besra. School of Biosciences, University of Birmingham

A distinct profile of cytokine production correlates with and predicts BCG induced protection in a Mycobacterium bovis murine challenge model
Daryan A. Kaveh, Elihu Aranday-Cortes and Philip J. Hogarth, TB Research Group, Animal Health and Veterinary Laboratories Agency, Weybridge, Surrey

Insights into immigrant screening for tuberculosis from evaluation of practice and a multi-centre cohort study of cost-effectiveness
Manish Pareek, John P. Watson, L Peter Ormerod, Onn Min Kon, Gerrit Woltmann, Peter J White, Ibrahim Abubakar, Geoffrey P Garnett, Ajit Lalvani, Tuberculosis Research Unit, National Heart and Lung Institute, Imperial College London, UK & Department of Infectious Disease Epidemiology, Imperial College London, UK

AFC Winter 2011 meeting

AFC Winter 2011

Held at St George’s University of London

January 7th 2011

Scientific Programme

Session 1

Tandem duplications of large genomic islands in Mycobacterium avium subspecies paratuberculosis associated with human strains isolated from patients with Crohn’s disease
Tim Bull, Richard Linedale, Wojtek Michalski, James Wynne. Department of Cellular and Molecular Medicine, Division of Clinical Sciences, St George’s, University of London

Functional analysis of the serine-threonine protein kinase, PknF, and its substrate the ABC transporter Rv1747, in Mycobacterium tuberculosis
Vicky L. Spivey, Virginie Molle, Rachael H. Whalan, Angela Rodgers, Apoorva Bhatt, Lasse Stach, Elizabeth M. Hirst, Gurdyal S. Besra, K. Barry Walker, Stephen J. Smerdon, Roger S. Buxton. MRC National Institute for Medical Research

Identification of biomarkers of treatment response in blood by transcriptomic analysis
Jackie Cliff, Ji-Sook Lee, Nicholas Constantinou, Jang-Eun Cho, Taane Clark, Katharina Ronacher, Paul van Helden, Gerhard Walzl, Hazel Dockrell. Department of Immunology and Infection, London School of Hygiene & Tropical Medicine

Gene products HsaD and HsaC in cholesterol metabolism of Mycobacterium tuberculosis
M. Abdul Aziz, N. Lack, R. Ballet, V. Cheng, N. Laurieri, S. Keany, A. Ryan, & E. Sim. Department of Pharmacology, University of Oxford

Lung heparin targeted vaccine delivery of mycobacterial antigens
Elena Stylianou, Ilaria Pepponi, Craig van Dolleweerd, Julian Ma and Rajko Reljic Division of Clinical Sciences, St George’s, University of London

Session 2

Immunological responses following BCG vaccination and Mycobacterium bovis challenge in the Eurasian badger
Roland Ashford, Sandrine Lesellier, Si Palmer, Sonya Gowtage, Deanna Dalley, Dipesh Dave, Laura Chamberlain, Benjamin Grundy, R.Glyn Hewinson, Brian Catchpole, Frank E. Aldwell, and Mark Chambers. Veterinary Laboratories Agency

Mycobacterium tuberculosis acg in the hspX gene cassette is required for growth and virulence in vivo
Yanmin Hu and Anthony R. M. Coates. Centre of Infection and Immunity, Division of Clinical Sciences, St George’s University of London

Identification of novel non-tuberculous mycobacterial antigens
A Checkley, D Wyllie, I Satti, T Scriba, H Fletcher, A Hill, W Hanekom, H McShane. Jenner Vaccine Institute, Oxford University

Analysis of M. tuberculosis strains from the North London isoniazid resistant outbreak shows independent evolution of drug resistance
R.J. Shorten, A. McGregor, S.H. Gillespie, C. Jenkins, S. Platt and T.D. McHugh. Department of Medical Microbiology, Royal Free Hospital, London & Centre for Clinical Microbiology, University College London

Differential small RNA expression across Mycobacterium bovis field strains
P. Golby, J. Nunez, J. Hinds, A. Witney, S. Bentley, G. Hewinson and S. Gordon. Veterinary Laboratories Agency

AFC Summer 2010 meeting

AFC Summer 2010

Held at the Veterinary Laboratories Agency, Weybridge

July 2nd 2010

Scientific Programme

Session 1

Mycobacterium bovis Mini-symposium
Glyn Hewinson (VLA).

Bovine Tuberculosis (bTB) is the most important animal health problem facing the country today. This mini symposium will outline the scientific advances made in understanding the spread of bTB and in developing new tools for its control. A question and answer session will allow members to discuss the current scientific evidence base and what can be concluded from it.

Session 2

Mycobacterium bovis-BCG vaccination induces specific transcriptome biosignatures in mice predicting vaccination success
Elihu Aranday-Cortes, Daryan Kaveh, Javier Nunez-Garcia, Philip J Hogarth, H. Martin Vordermeier Veterinary Laboratories Agency

Towards replicating the transcriptome of Mycobacterium tuberculosis in sputum by exposure to different in vitro conditions
Su-Min Lee, Natalie Garton and Mike Barer. University of Leicester

Mycobacterium tuberculosis RD1 is a requisite for virulence-associated cellular recruitment
Suzie Hingley-Wilson and Ajit Lalvani. Wright Fleming Institute of Infection & Immunity, Imperial College London

Session 3

Mycobacterium tuberculosis SigG does not control expression of DNA repair genes in response to DNA damage
Katherine L. Smollett, Lisa F. Dawson and Elaine O. Davis. MRC National Institute for Medical Research, London

Matrix metalloproteinase-1 causes tissue destruction in tuberculosis
Paul Elkington, Takayuki Shiomi, Ronan Breen, Robert K Nuttall, Cesar Augusto Ugarte-Gil, Naomi Walker, Luísa Saraiva, Bernadette Pedersen, Francesco Mauri, Marc Lipman, Dylan R Edwards, Brian D Robertson, Jeanine D’Armiento, & Jon S Friedland. Department of Infectious Diseases and Immunity, Imperial College London

Rapid and efficient construction of mycobacterial mutagenesis vectors using ligation-independent cloning
Ricardo J. C. Balhana, Neil G. Stoker, Sharon L. Kendall. The Royal Veterinary College, London.

Enumeration of polyfunctional antigen-specific T cells by fluorescence-linked-immunospot identifies immune signatures that correlate with distinct stages of tuberculosis infection
Rosalyn Casey, Deena Blumenkrantz, Kerry Millington, Damien Montamat-Sicotte, Onn Min Kon, Melissa Wickremasinghe, Samuel Bremang, Murphy Magtoto, Saranya Sridhar, David Connell & Ajit Lalvani. National Heart and Lung Institute, Imperial College London

New tuberculosis drugs targeted to the essential kinase, PknB
Kathryn E. A. Lougheed, Simon Osborne, Tim Chapman, Barbara Saxty, David Whalley, Tim Nott, Steve Smerdon, Dony Patel, Jasveen Chugh, Catherine Kettleborough, Justin Bryans, Debbie Taylor, Roger S. Buxton. MRC National Institute for Medical Research Mill Hill, London.

Expression and localisation of MPM70 during infection: evidence for a post-phagosomal role in pathogenesis
Alistair F. Ray, Kornelis R. Straatman, Lorna C. Waters, Phillip S. Renshaw, Kirsty L. Lightbody and Mark D. Carr. University of Leicester

AFC Winter 2010 meeting

AFC Winter 2010

Held at the London School of Hygiene & Tropical Medicine

January 8th 2010

Scientific Programme

Session 1

Pea Production by mycobacteria
Ruth McNerney and Claire Turner. Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine.

The Mycobacterium tuberculosis HigA antitoxin does not directly regulate any other genes in addition to its own operon
Amanda S. Fivian-Hughes and Elaine O. Davis. Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

The regulation of intracellular iron in the BCG-vaccinated host response to M. tuberculosis infection as determined by microarray
Ruth Thom, JA Tree, SO Clark, MJ Elmore, A Williams, PD Marsh. Centre for Emergency Preparedness and Response, Health Protection Agency, Porton Down, Salisbury, Wiltshire, SP4 OJG, UK

A salicylate-inducible promoter of Mycobacterium tuberculosis
Dorothee Schuessler, Amanda Brown and Tanya Parish. Queen Mary University of London, Barts and the London School of Medicine and Dentistry. CIID BICM 4, Newark Street London E1 2AT

Investigation of the stability of mycobacterial ribosomes during active growth and stasis
Andrej Trauner and Huw D. Williams. Department of Life Sciences, Imperial College, London

Session 2

Tribute to Chris Collins
given by Malcolm Yates

Human Natural Killer cell activity in response to M. bovis BCG versus M. tuberculosis H37Rv
Damien Portevin & Douglas Young. NIMR, London, UK

The Universal Stress Protein Rv1636 from Mycobacterium tuberculosis is a cation independent nucleotide triphosphatase
Dominic Bradley, Sergio Leiva, Simon Pennell, Ian A. Taylor, Stephen J. Smerdon, Huw D. Williams. Department of Life Sciences, Faculty of Natural Sciences, Imperial College, London

Granulocytes are necessary for the detection of mycobacteria-specific CD8+ T-cells responses in healthy individuals
Iman N. Satti, Natalie E.R. Beveridge, Helen A. Fletcher, Ansar A. Pathan, Thomas J. Scriba, Angela Minassian, Clare R. Sander, Willem A. Hanekom,Adrian V.S. Hill and Helen McShane. Jenner Institute, Old Road Campus Research Building, Nuffield Department of Medicine, Oxford University, Churchill Hospital, Old Road, Oxford, UK, OX3 7D

Optimisation of bioluminescent reporter constructs for in vivo imaging of mycobacteria
Nuria Andreu, Andrea Zelmer, Taryn Fletcher, Paul T. Elkington, Siouxsie Wiles and Brian D. Robertson. Department of Microbiology, Imperial College London, Flowers Building, Exhibition Road, London, SW7 2AZ, UK

AFC Summer 2009 meeting

AFC Summer 2009

Held at the Moredun Research Institute, Edinburgh

July 2nd-3rd 2009

Scientific Programme

Session 1: Research at the Moredun

Johne’s disease: an overview of research at Moredun
Karen Stevenson, Valerie Hughes, Craig A Watkins, Linda May, Susan Denham, Ian Heron and Joyce McLuckie:

Corynebacterium pseudotuberculosis – from genome to prevention
Florence E Pethick, Alex Lainson, Michael C Fontaine

Session 2

Phenotype microarray platform: Potential and pitfalls for use with pathogenic mycobacteria
Bhagwati Uphadyay and Paul R Wheeler. Veterinary Laboratories Agency, Weybridge

Fast growing, non-infectious and intra-cellularly surviving drug-resistant Mycobacterium aurum: A model for high-throughput anti-TB drug screening
Antima Gupta, Sanjib Bhakta, Subir Kundu, Manish Gupta, Brahm S. Srivastava, and Ranjana Srivastava. Central Drug Research Institute, India & Birkbeck College, University of London.

Kinetic Profiling of Immune responses to PE/PPE proteins in M. bovis-infected Cattle
H.M. Vordermeier, R.G. Hewinson, D.B. Young, S.L. Sampson. VLA Weybridge& Imperial College London

Session 3

Life and death in the mycobacterial phagosome
Rachel Shrimpton, Priscille Brodin, Olivier Neyrolles, Graham Stewart. Division of Microbial Sciences, University of Surrey, UK

Identification of 2-aminothiazole—4-carboxylate derivatives active against Mycobacterium tuberculosis H37Rv and the ketoacyl-ACP synthase mtFabH
Qosay Al-Balas, Nahoum G. Anthony, Bilal Al-Jaidi, Amani Alnimir, Grainne Abbott, Alistair K. Brown, Rebecca C. Taylor, Gurdyal. S. Besra, Timothy D. McHugh, Stephen H. Gillespie, Blair F. Johnston, Simon P. Mackay, Geoffrey D. Coxon. University of Strathclyde, Glasgow.

BCG: surrogate for a human TB challenge?
Minassian, A, Tchilian, E, Poulton, I, Fletcher, H, Andrews, L,   Poyntz, H, Ronan, E,   Hill, A, & McShane, H. The Jenner Institute, Oxford University

Acute and persistent Mycobacterium tuberculosis infections depend on the thiol peroxidase Tpx
Yanmin Hu and Anthony R. M. Coates. St George’s University of London.


Genes and proteins of the nat/hsaD gene cluster and their role in cholesterol metabolism
Nathan Lack, Dimitrios Evangelopoulos, Sanjib Bhakta, Lindsay Eltis, Areej Abuhammad, Elizabeth Fullam, Edward Lowe and Edith Sim. University of Oxford.

Session 4

Structure and functional characterisation of Mycobacterium tuberculosis diaminopimelic acid epimerase (MtDapF)
Veeraraghavan Usha, Lynn G. Dover, Klaus Fütterer, David L. Roper, Adrian J. Lloyd and Gurdyal S. Besra. University of Birmingham

The helicase UvrD2 is essential in Mycobacterium tuberculosis
Alan Williams, Burkhard Springer, Erik C. Böttger, and Elaine O. Davis. NIMR, Mill Hill, London

Dissection of regenerating T cell responses against tuberculosis in HIV infected adults with latent tuberculosis
Katalin A. Wilkinson, Ronnett Seldon, Graeme Meintjes, Molebogeng X. Rangaka, Willem A. Hanekom, Gary Maartens, Robert J. Wilkinson. University of Cape Town, South Africa

Live cell imaging to study growth and cell division in mycobacteria
Graham Joyce, Kerstin J. Williams, and Brian D. Robertson. Imperial College London

AFC Winter 2009 meeting

AFC Winter 2009

Held at the NIMR, Mill Hill

January 9th 2009

Scientific Programme

Session 1

Identification of small RNAs in Mycobacterium tuberculosis
Kristine B. Arnvig and Douglas B. Young. NIMR, London

Identification of TB contacts with latent TB infection in a population with a high HIV and TB burden
Kerry A Millington,Junior Mutsvangwa, Kathryn Chaka, Tafadzwa Mavhudzi, Yin-Bun Cheung, Peter R Mason, , Anthony E Butterworth, Elizabeth L Corbett and Ajit Lalvani. Imperial College, London

Characterisation of a putative dihydrofolate reductase from Mycobacterium smegmatis
Dimitrios Evangelopoulos, Nathan Lack, Giles Robertson, Tina Daviter, Nicholas H. Keep, Edith Simand Sanjib Bhakta. Birkbeck College, University of London

Changes in T cell population dynamics in 3 species: Dynamics of white cell populations in M. tuberculosis infection in rhesus macaques
K Gooch, S Sharpe, A Williams, M Dennis, P D Marsh. Health Protection Agency, CEPR, Porton Down

Detection and molecular characterization of 9000-year old Mycobacterium tuberculosis from a Neolithic settlement in the Eastern Mediterranean
Oona Lee, David Minnikin, Gurdyal Besra, Israel Hershkovitz, Vered Eshed, Ehud Galili, Charles Greenblatt, Eshetu Lemma, Gila Kahila Bar-Gal, Mark Spigelman, Helen Donoghue. University of Birmingham

Session 2

Inhibition of phago-lysosome fusion by Mycobacterium bovis BCG induces a Niemann-Pick type C1-like phenotype
Emyr Lloyd-Evans, Nathan Lack, Edith Sim and Frances M. Platt. University of Oxford

KstR2: Identification of a second TetR-type regulator involved in cholesterol catabolism in mycobacteria
Sharon Kendall, Annemieke ten Bokum, Phillipa Burgess, Mike Withers, Iria Uhia Castro, Nwaamaka Owoh, Nadia Mokhtar and Neil Stoker. The Royal Veterinary College, London

Intranasal vaccination with an ESAT-6 expressing attenuated influenza vaccine
A. Egorov. AVIR Green Hills Biotechnology, Vienna

BCG vaccination induces polyfunctional CD4+ T cells in the spleen which correlate with active immunity to a Mycobacterium bovis challenge
Philip J Hogarth. Veterinary Laboratories Agency, Weybridge.

The Burden of Mycobacterial Disease in Ethiopian Cattle
Stefan Berg, Rebuma Firdessa, Endalamaw Gadisa, Meseret Habtamu, Araya Mengistu, Lawrence Yamuah, Gobena Ameni, Brian D. Robertson, Douglas B. Young, Howard Engers, Noel H. Smith, Martin Vordermeier, R. Glyn Hewinson, Abraham Aseffa, and Stephen V. Gordon. Veterinary Laboratories Agency, Weybridge

AFC Summer 2008 meeting

AFC Summer 2008

Held at the University of Leicester

July 10th-11th 2008

Scientific Programme

Session 1: Leicester-related symposium Part 1
Overview of TB research at Leicester
Mike Barer

Short read sequencing of Mycobacterium tuberculosis
NJ Loman, GM Weinstock, K Rajakumar, MR Barer & M Pallen.

Structural and Functional Characterisation of Complexes Formed by ESAT-6/CFP-10 Family Proteins
Kirsty Lightbody, Dariush Ilghari, Lorna Waters, Vaclav Veverka, Richard Williamson,Philip Renshaw and Mark Carr

The role of PknG in metabolic regulation in M. tuberculosis
Helen O’Hare, Rosario Durán, Jens Baumgartner, Marco Bellinzoni, Anne-Marie Wehenkel, Carlos Cervenansky, Pedro Alzari, Kai Johnsson

Session 2: Leicester-related symposium Part 2
Studies on lipid bodies of Mycobacterium tuberculosis: Survival of the fattest?
Su-Min Lee, Galina Mukamolova, Anna Sherratt, Natalie Garton and Mike Barer

TBIT: A Database for the integrated surveillance and management of tuberculosis cases, latent Tb infection (LTBI) and microbiology
Gerrit Woltmann, Rob Free, Joanne Malkin and Mike Barer.

Evaluation of mask sampling and rapid molecular assays in studying the transmission of tuberculosis in Leicester
Eddy Cheah, Joanne Malkin, Rob Free and Mike Barer

Session 3: Offered papers

The Mtb Cytochrome P450 Systems: structural insights and drug targeting
Kirsty J McLean, Colin Levy, David Leys and Andrew W Munro, University of Manchester

A simple mutation-based assay for determining the translational start sites of mycobacterial proteins
Katherine Smollett, Amanda Fivian-Hughes, Anchi Chang and Elaine Davis, MRC National Institute for Medical Research, London

Relationship of Th1 and FoxP3 positive T cells to the HIV-tuberculosis immune reconstitution inflammatory syndrome
Graeme A Meintjes, Katalin Wilkinson, Molebogeng Rangaka, Keira Skolimowska, Kerryn van Veen, Musaed Abrahams,Ronnett Seldon, Priscilla Mouton, Dominique J Pepper, Kevin Rebe, Gilles van Cutsem, Mark Patrick Nicol, Gary Maartens, Robert Wilkinson, University of Cape Town, South Africa

Characterization of a mycobacterial strain that fails to produce mature mycolic acids
Apoorva Bhatt, University of Birmingham

Novel Antimycobacterial Agents Targeting Mycolate Synthesis: Are They Pyrazinamide Like?
Amani Alnimr, Geoff Coxon, Tim McHugh, Stephen Gillespie. Royal Free and University College Medical School

Session 4: Offered papers

Oral BCG-Lipid protects from aerogenic M. bovis infection in the guinea pig
Simon Clark, Frank Aldwell, Martin Cross, Julia Vipond, Yper Hall, Allan Nadian, Ann Williams, Mark Chambers, HPA, CEPR, Porton

Identification of a crucial branching enzyme in mycobacterial arabinogalactan biosynthesis
Luke J. Alderwick, Helen L. Birch, Apoorva Bhatt, Doris Rittmann, Karin Krumbach, Albel Singh, Yu Bai, Todd L. Lowary, Lothar Eggeling, and Gurdyal S. Besra, University of Birmingham

MurE, an ATP dependent ligase in Mycobacterium tuberculosis: recombinant expression, purification and biochemical characterisation
Chandrakala Basavannacharya, Giles Robertson, Alexis Rubio, Edith Sim, Nicholas Keepand Sanjib Bhakta, Birkbeck, University of London

Genetic requirements for growth rate modulation during continuous culture in a chemostat
Dany JV Beste, Bhushan Bonde, Graham Stewart, Claudio Avignone-Rossa, Mike Bushell, Andrzej Kierzek and Johnjoe McFadden, University of Surrey, Guildford

AFC Winter 2008 meeting

AFC Winter 2008

Held at Barts & The London

January 11th 2008

Scientific Programme

Session 1
Validating drug targets in Mycobacterium tuberculosis
David M. Roberts, Paul Carroll, Karen Adie, Tanya Parish. Infectious Disease Research Institute, Seattle, USA

Unique transcriptome profiles of M.tuberculosis in human sputum
Simon Waddell, Natalie Garton, Claire Senner, Jason Hinds, Mike Barer and Philip Butcher. St George’s University of London

Dissecting M.tuberculosis driven signalling pathways in human macrophages causing tissue destruction
L. Rand, J.A. Green, P.T.G. Elkington, J. S. Friedland. Infectious Diseases and Immunity, Imperial College London

Rapid differentiation of rifampicin-resistant strains of Mycobacterium tuberculosis
Kim Mallard, Ghada S. Sharaf Eldon and Ruth McNerney. London School of Hygiene and Tropical Medicine

Heterogeneity of HupB, an iron-regulated protein of mycobacteria
Veena C Yeruva, Paul R. Wheeler, Stephen Gordon and Manjula Sritharan. School of Life Sciences, University of Hyderabad, India

Session 2

Bystander macrophage apoptosis after Mycobacterium tuberculosis H37Ra infection
Joseph Keane, Annemieke M.C. ten Bokum, Seonadh M. O’Leary, Deirdre M. Kelly, Mary P. O’Sullivan. Trinity College Dublin and St. James’s Hospital, Dublin, Ireland

Analysis of a single nucleotide polymorphism in M. bovis BCG
Debbie M. Hunt, José W. Saldanha, John F. Brennan, Pearline Benjamin, Jeffrey A. Cole, Claire L. Spreadbury and Roger S. Buxton. MRC National Institute for Medical Research.

Hypervirulence through gene deletion in Mycobacterium tuberculosis
Annemieke ten Bokum, Farah Movahedzadeh, Rosangela Frita, Sharon Kendall, Debbie Smith, Greg Bancroft and Neil Stoker. London School of Hygiene & Tropical Medicine

Deletion of hmp gene alters lipid biosynthesis of M. tuberculosis and leads to attenuation of the organism in macrophage and murine infection models
Yanmin Hu1, Gurdyal S. Besra,2 Sudagar S. Gurcha,2 Manfred Rohde3  Mahavir Singh3 and Anthony Coates1. 1 St George’s University of London, 2 School of Biosciences, The University of Birmingham, 3 Helmholtz Centre for Infection Research, Braunschweig, Germany

Survival of Mycobacterium bovis in protozoa
Cornelia Mardare, Frans de Leij & Jeremy W Dale. University of Surrey, Guildford